abstract：:A single dose of the carcinogen aflatoxin B1 (7 mg/kg body weight) to male Wistar rats significantly enhanced the hepatic activity of protein kinase C in the particulate and nuclear fractions. The particulate fraction showed stimulation at 4 and 7 h, while the nuclear activity was increased at 17 h following administr...

journal_title：Chemico-biological interactions

authors： Mistry KJ,Krishna M,Pasupathy K,Murthy V,Bhattacharya RK

更新日期：1996-03-25 00:00:00

abstract：:2-Nitropropane (2-NP) is a genotoxicant and hepatocarcinogen in rodents. Conversion to propane 2-nitronate (P2N), the anion of the tautomeric aci form of 2-NP, seems to be a pivotal part of the mechanism by which 2-NP causes its toxicity. We tested the hypothesis that the tautomeric equilibrium is influenced by enzyme...

journal_title：Chemico-biological interactions

authors： Kohl C,Gescher A

更新日期：1996-01-05 00:00:00

abstract：:Exposure to perfluorocarboxylic acids, pthalate esters, and some hypolipidemic agents results in the proliferation of peroxisomes in the rodent liver. The structural diversity of these compounds suggests mechanistic diversity in their toxicity as well. To establish reliable biomarkers of peroxisome proliferation (PP) ...

journal_title：Chemico-biological interactions

authors： Witzmann F,Coughtrie M,Fultz C,Lipscomb J

更新日期：1996-01-05 00:00:00

abstract：:Chronic exposure of humans to benzene causes severe bone marrow cell depression leading to aplastic anemia. Marrow stromal macrophage dysfunction and deficient interleukin-1 production has been reported for patients with severe aplastic anemia. The stromal macrophage, a target of benzene toxicity, is involved in hemat...

journal_title：Chemico-biological interactions

authors： Niculescu R,Bradford HN,Colman RW,Kalf GF

更新日期：1995-12-22 00:00:00

abstract：:Vitamin A-deficient male mice were treated for 10 days with 0.02% perfluorooctanoic acid (PFOA) in their diet. The effects of this highly potent peroxisome proliferator on peroxisomal palmitoyl-CoA oxidation and lauroyl-CoA oxidase activities were reduced by 3.1-7.5 times in comparison to the values obtained with mice...

journal_title：Chemico-biological interactions

authors： Sohlenius AK,Andersson K,Olsson J,DePierre JW

更新日期：1995-10-20 00:00:00

abstract：:Treatment of a lysosome-rich fraction from liver with 2-chloroethylethyl sulfide resulted in a dose-dependent release of arylsulfatase. The inclusion of Ca2+ enhanced the enzyme release by approximately 2.3-fold. The enhancing effect of Ca2+, showing an EC50 value of 30 mM, was mimicked by neither Mg2+ nor Mn2+. Studi...

journal_title：Chemico-biological interactions

authors： Shin S,Choi DS,Kim YB,Cha SH,Sok DE

更新日期：1995-08-18 00:00:00

abstract：:This study investigated the effect of inducers on the major enzymes responsible for metabolising the quinone antitumor agent mitoxantrone, and on its cytotoxicity in MCF 7 human breast cancer cells. Four inducers were used: 1,2-benzanthracene (BA), phenobarbitone (PB); rifampicin (R) and dexamethasone (DEX). Of these,...

journal_title：Chemico-biological interactions

authors： Li SJ,Rodgers EH,Grant MH

更新日期：1995-07-14 00:00:00

abstract：:Studies were undertaken to investigate the mechanism of bioactivation and covalent binding of TNT. Incubation of [14C]TNT with rat liver microsomes in the presence of an NADPH generating system resulted in metabolism and covalent binding to microsomal proteins. Time-dependence studies showed that TNT was rapidly reduc...

journal_title：Chemico-biological interactions

authors： Leung KH,Yao M,Stearns R,Chiu SH

更新日期：1995-06-30 00:00:00

abstract：:The distribution and metabolism of the environmental pollutant 1-nitropyrene was studied in C57B1/6N mice following oral or intraperitoneal dosing. When administered by gavage, 1-nitropyrene and its metabolites demonstrated biphasic elimination kinetics from the blood, with half-lives of 0.3 and 1.8 days and a distrib...

journal_title：Chemico-biological interactions

authors： Howard PC,Consolo MC,Dooley KL,Beland FA

更新日期：1995-04-14 00:00:00

abstract：:The (+/-)-anti-dihydrodiol epoxides (DE) of benzo[a]pyrene (BP), chrysene (Chr), benzo[c]phenanthrene (BcPh) and dibenz[a,h]anthracene (DBA) were incubated in the presence of glutathione (GSH) with hepatic cytosol from untreated and Aroclor 1254 pretreated rats and with the Mu-class glutathione transferase (GST) HTP I...

journal_title：Chemico-biological interactions

authors： Funk M,Gath I,Seidel A,Oesch F,Platt KL

更新日期：1995-03-30 00:00:00

abstract：:The extent of DNA modification in cancerous rat live and lung tissues was investigated and compared to their respective normal tissues. Liver tumors were induced by 2-fluorenylacetamide (2-FAA) or N-nitroso-N-2-fluorenylacetamide (N-NO-2-FAA), and lung tumors were induced by sodium nitrite plus trimethylamine. In the ...

journal_title：Chemico-biological interactions

authors： Wang YJ,Ho YS,Lo MJ,Lin JK

更新日期：1995-02-01 00:00:00

abstract：:The in vivo metabolite patterns of 2,5-difluoroaminobenzene and of its nitrobenzene analogue, 2,5-difluoronitrobenzene, were determined using 19F NMR analysis of urine samples. Results obtained demonstrate significant differences between the biotransformation patterns of these two analogues. For the aminobenzene, cyto...

journal_title：Chemico-biological interactions

authors： Rietjens IM,Cnubben NH,van Haandel M,Tyrakowska B,Soffers AE,Vervoort J

更新日期：1995-01-01 00:00:00

abstract：:The knowledge of brain metallothionein (MT) regulation and especially of MT presence in specific cell types is scarce. Therefore, the effect of several well-known MT inducers, measured by radioimmunoassays using antibodies that cross-react with MT-I and MT-II or specific for MT-I and which do not cross-react with huma...

journal_title：Chemico-biological interactions

authors： Hidalgo J,García A,Oliva AM,Giralt M,Gasull T,González B,Milnerowicz H,Wood A,Bremner I

更新日期：1994-12-01 00:00:00

abstract：:Dopa was oxidized by Mn(3+)-pyrophosphate complex to the corresponding o-quinone, accompanied by the cyclization of the amino chain to form cyclized dopa ortho-quinone (cDoQ) with absorption maxima at wavelengths of 305 and 475 nm. The cyclization was found to proceed in a single step from DoQ to cDoQ without formatio...

journal_title：Chemico-biological interactions

authors： Baez S,Linderson Y,Segura-Aguilar J

更新日期：1994-11-01 00:00:00

abstract：:The cytostatic drug 6-mercaptopurine riboside (6-MPr) was investigated in mice in order to test the hypothesis that the teratogenicity of this antimetabolite is paralleled by an incorporation into the DNA of the embryos during organogenesis. DNA modification in the embryos was analysed 4 h following s.c. administratio...

journal_title：Chemico-biological interactions

authors： Platzek T,Schwabe R,Rahm U,Bochert G

更新日期：1994-10-01 00:00:00

abstract：:Neutrophils from asymptomatic HIV-infected patients have an increased Nitroblue tetrazolium (NBT) reduction, that is an increased production of oxygen radicals. Plasma from these patients can activate normal neutrophils to an increased NBT-reduction and the neutrophil activating factor thus seems to be mainly plasma b...

journal_title：Chemico-biological interactions

authors： Jarstrand C,Akerlund B

更新日期：1994-06-01 00:00:00

abstract：:Rat hepatic hydroxysteroid sulfotransferase with sulfoconjugates androsterone (androsterone-sulfating sulfotransferase) is an oligomer consisting of several subunits with distinct pI values but with the same molecular mass (pI variants). N-terminal amino acid sequences of the pI variants are all identical. The enzyme ...

journal_title：Chemico-biological interactions

authors： Homma H,Nakagome I,Kamakura M,Hirota M,Takahashi M,Matsui M

更新日期：1994-06-01 00:00:00

abstract：:Cloning of the androgen and estrogen sulfotransferases has allowed studies on their spatiotemporal regulation and physiological function. These two enzymes appear to be involved in regulating target cell sensitivity for the androgenic and estrogenic steroids. Recombinant androgen sulfotransferase produced in insect Sf...

journal_title：Chemico-biological interactions

authors： Chatterjee B,Song CS,Kim JM,Roy AK

更新日期：1994-06-01 00:00:00

abstract：:The cDNA for human liver phenol-sulfating phenol sulfotransferase (P-PST) has been cloned and the active enzyme expressed in Cos cells and bacteria. Analysis of the sequence identified two cysteine residues, one of which is highly conserved in the phenol sulfotransferase gene family. Previous studies with the pure hum...

journal_title：Chemico-biological interactions

authors： Falany CN,Zhuang W,Falany JL

更新日期：1994-06-01 00:00:00

abstract：:Due to its antiviral effects and its importance for all immunological functions, the administration of selenium is suggested as a supportive measure in early as well as in advanced stages of HIV-induced disease. Initial observations on the effects of selenium supplementation in HIV-infected patients indicate that sele...

journal_title：Chemico-biological interactions

authors： Schrauzer GN,Sacher J

更新日期：1994-06-01 00:00:00

abstract：:Multiplicity of hepatic microsomal coenzyme A ligases catalyzing acyl-CoA thioester formation is an important factor for consideration in relation to the metabolism of xenobiotic carboxylic acids. In this study the kinetic characteristics of rat hepatic microsomal nafenopin-CoA ligase were studied and compared with th...

journal_title：Chemico-biological interactions

authors： Knights KM,Roberts BJ

更新日期：1994-03-01 00:00:00

abstract：:Isolated rat hepatocytes were used to investigate the biochemical mechanisms of toxicity of triethyllead (Et3Pb+), a highly neurotoxic degradation product of the antiknocking petrol additive tetraethyllead. As early as 5 min from the addition of 50 microM Et3Pb+ to hepatocyte suspensions a decrease of mitochondrial me...

journal_title：Chemico-biological interactions

authors： Albano E,Bellomo G,Benedetti A,Carini R,Fulceri R,Gamberucci A,Parola M,Comporti M

更新日期：1994-01-01 00:00:00

abstract：:Disodium cromoglycate (DSCG) in equimolar concentration of 0.56 mumol/l preincubated with an asynchronous cell population decreases the inhibition of proliferation and results in a 100% elimination of inhibition of colony formation induced by benfluron (BF). DSCG protects the cells from unbalanced growth and unbalance...

journal_title：Chemico-biological interactions

authors： Horáková K,Vlcková A,Lukácová D

更新日期：1993-12-01 00:00:00

abstract：:The role of catecholamines in the toxicity of MPTP (N-methyl-4-phenyl- 1,2,3,6-tetrahydropyridine) was explored. The killing of cultured hepatocytes by dopamine and 6-hydroxydopamine was enhanced following inhibition of glutathione reductase by 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU), a manipulation known to sensi...

journal_title：Chemico-biological interactions

authors： Snyder JW,Alexander GM,Ferraro TN,Grothusen JR,Farber JL

更新日期：1993-09-01 00:00:00

abstract：:In this study, the presence of paraoxonase activity in pericardial fluid was demonstrated. A comparison of some properties, such as optimum pH, stability versus pH, heat inactivation, effect of inhibitors, isoelectric point and kinetic parameters (Km and Vmax), between plasma and pericardial fluid paraoxonase was made...

journal_title：Chemico-biological interactions

authors： Hernández AF,Pla A,Valenzuela A,Gil F,Villanueva E

更新日期：1993-06-01 00:00:00

abstract：:The hydrolysis of paraoxon (POX), phenylacetate (PA) and beta-naphthylacetate (BNA) was studied in human serum. Based upon correlations between enzyme activities, upon reversible inhibition by EDTA and upon progressive inhibition by iso-OMPA, tabun, eserine and bis-4 nitrophenylphosphate, the following conclusions wer...

journal_title：Chemico-biological interactions

authors： Reiner E,Pavković E,Radić Z,Simeon V

更新日期：1993-06-01 00:00:00

abstract：:Glucosamine oligomers--monomer through tetramer--form complexes with Cu2+ that catalyse the hydrolysis of the 'nerve gas' 1,2,2-trimethylpropyl- methylphosphonofluroidate (soman) by cleaving the P-F bond. A 1/1 glucosamine/Cu2+ ratio whether as glucosamine or glucosamine units, gives the highest hydrolytic rate over t...

journal_title：Chemico-biological interactions

authors： Hoskin FC,Rajan KS,Remy D,Walker JE

更新日期：1993-06-01 00:00:00

abstract：:The kinetics of time- and concentration-dependent covalent organophosphorus inhibition of carboxylesterase isoenzymes (EC 3.1.1.1) and cholinesterase isoenzymes (EC 3.1.1.7 and EC 3.1.1.8) were investigated using a wide range of organophosphate inhibitor concentrations (10(-10)-10(-3) mol/l) and different inhibition t...

journal_title：Chemico-biological interactions

authors： Chemnitius JM,Dewald K,Kreuzer H,Zech R

更新日期：1993-06-01 00:00:00

abstract：:Avian serum esterases are predominantly of the 'B' type (cholinesterases and carboxylesterases) and are inhibited by carbamates and the 'active' oxon forms of organophosphorus pesticides. Selective inhibition of mammalian serum carboxylesterase, a 'B' esterase, has shown that this enzyme may play an important role in ...

journal_title：Chemico-biological interactions

authors： Thompson HM

更新日期：1993-06-01 00:00:00

abstract：:O-Ethyl-O-4-nitrophenylphosphoramidate is a short-acting anticholinesterase and a possible candidate for a prophylactic agent against nerve agents since human acetylcholinesterase inhibited by this agent undergoes rapid spontaneous reactivation which can be accelerated further, if necessary, by treatment with oximes. ...

journal_title：Chemico-biological interactions

authors： Johnson MK,Read DJ

更新日期：1993-06-01 00:00:00

abstract：:Quinone methides are a class of reactive, electrophilic compounds which are capable of alkylating cellular macromolecules. They are formed during xenobiotic biotransformation reactions and are hypothesized to mediate the toxicity of a large number of quinone antitumor drugs as well as several alkylphenols. In addition...

journal_title：Chemico-biological interactions

authors： Thompson DC,Thompson JA,Sugumaran M,Moldéus P

更新日期：1993-02-01 00:00:00

abstract：:A new, direct method for determining superoxide dismutase activity is presented in this study. This method is based on measurement of one of the products of the superoxide dismutase reaction, hydrogen peroxide. Hydrogen peroxide is quantitated using a coupled reaction where horseradish peroxidase catalyzes the formati...

journal_title：Chemico-biological interactions

authors： Segura-Aguilar J

更新日期：1993-01-01 00:00:00

abstract：:The effects of pH on access to the cytochrome P-450 active site, N-demethylation and formation of the cytochrome P-450 Fe(II)-RNO metabolite complex for a series of erythromycin derivatives were examined. Studies were performed with dexamethasone-treated rat liver microsomes containing large amounts of cytochrome P-45...

journal_title：Chemico-biological interactions

authors： Delaforge M,Ladam P,Bouillé G,Benarous JG,Jaouen M,Girault JP

更新日期：1992-12-01 00:00:00

abstract：:The effects of dietary treatment with clofibrate (0.5% w/w for 10 days) on the livers of selenium-deficient male rats were examined. The peroxisome proliferation (as determined by electron microscopy) in the livers of selenium-deficient animals was much less pronounced than in the case of selenium-adequate rats and no...

journal_title：Chemico-biological interactions

authors： Olsson U,Garberg P,Lundgren B,Andersson K,Hultenby K,Bergstrand A,Eriksson AM,Högberg J,DePierre JW

更新日期：1992-11-30 00:00:00

abstract：:Treatment of isolated mitochondria from rat hepatoma tumor cells (AS-30D) with the oxidant, t-butyl hydroperoxide (tBuOOH, 1 or 5 mumol/ml) resulted in the oxidation of glutathione (GSH to GSSG) and the formation of protein-glutathione mixed disulfides (ProSSG). The GSSG was retained inside of the hepatoma mitochondri...

journal_title：Chemico-biological interactions

authors： Brodie AE,Reed DJ

更新日期：1992-09-28 00:00:00

abstract：:1. The cytotoxicity of N-nitrosomethylaniline (NMA) towards hepatocytes isolated from rats was prevented by acetone or ethanol (inhibitors for cytochrome P-450IIE1) but not by metyrapone or SKF525A (inhibitors for cytochrome P-450IIB1/2). Various alcohols, secondary ketones and isothiocyanates that induced cytochrome ...

journal_title：Chemico-biological interactions

authors： Quan Z,Khan S,O'Brien PJ

更新日期：1992-08-28 00:00:00

abstract：:The mechanisms involved in the cytotoxicity of 2-bromo-3-(N-acetylcystein-S-yl)hydroquinone, a model compound for hydroquinone derived mercapturic acids, were investigated in rat renal proximal tubule cells. 2-Bromo-3-(N-acetylcystein-S-yl)hydroquinone induced a time- and concentration-dependent decrease in cell viabi...

journal_title：Chemico-biological interactions

authors： Vamvakas S,Bittner D,Koob M,Glück S,Dekant W

更新日期：1992-08-14 00:00:00

abstract：:The combined effects of ethanol and hypoxia on the conversion of xanthine dehydrogenase (D form) to xanthine oxidase (O form) and on the leakage of the enzyme from isolated rat hepatocytes was studied. Time-dependent death of cells occurred during incubation in hypoxic conditions. Ethanol (40 mM) had only a moderate e...

journal_title：Chemico-biological interactions

authors： Battelli MG,Abbondanza A,Stirpe F

更新日期：1992-06-15 00:00:00

abstract：:The organ specificity of tris(2,3-dibromopropyl)phosphate(Tris-BP)-induced DNA damage was investigated in the rat 2 h after a single i.p. injection of 350 mumol/kg. Extensive DNA damage, measured with the alkaline elution method, was found in the kidney, liver and small intestine. Less, but significant DNA damage was ...

journal_title：Chemico-biological interactions

authors： Søderlund EJ,Brunborg G,Dybing E,Trygg B,Nelson SD,Holme JA

更新日期：1992-04-15 00:00:00

abstract：:Aromatic amines are important intermediates in industrial manufacturing. N-oxidation to the N-hydroxyarylamines is a key step determining the genotoxic properties of aromatic amines. N-hydroxyarylamines can form adducts with DNA, with tissue proteins and with the blood proteins albumin and hemoglobin in a dose-depende...

journal_title：Chemico-biological interactions

authors： Sabbioni G

更新日期：1992-01-01 00:00:00